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ppd0010 crop seq car mcherry2 vector  (Addgene inc)


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    Structured Review

    Addgene inc ppd0010 crop seq car mcherry2 vector
    Ppd0010 Crop Seq Car Mcherry2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppd0010+crop+seq+car+mcherry2+vector/pGSTag+BimL+(T56A)+(Plasmid+%2324253)/pmc12545207-245-4-6
    Average 93 stars, based on 2 article reviews
    ppd0010 crop seq car mcherry2 vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cloning:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: .. This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: The ligation product was amplified by PCR with primers gBlock-17-FWD and gBlock-18-REV, column purified, digested one more time with BsmBI-v2 and inserted into the CROP-seq backbone by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). .. This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_ CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.

    Plasmid Preparation:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: .. This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: The ligation product was amplified by PCR with primers gBlock-17-FWD and gBlock-18-REV, column purified, digested one more time with BsmBI-v2 and inserted into the CROP-seq backbone by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). .. This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_ CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.

    Derivative Assay:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: .. This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: The ligation product was amplified by PCR with primers gBlock-17-FWD and gBlock-18-REV, column purified, digested one more time with BsmBI-v2 and inserted into the CROP-seq backbone by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). .. This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_ CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.

    other:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
    Article Snippet: For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_ CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens.



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    ppd0010 crop seq car mcherry2 vector  (Addgene inc)
    93
    Addgene inc ppd0010 crop seq car mcherry2 vector
    Ppd0010 Crop Seq Car Mcherry2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppd0010+crop+seq+car+mcherry2+vector/pGSTag+BimL+(T56A)+(Plasmid+%2324253)/pmc12545207-245-4-6
    Average 93 stars, based on 1 article reviews
    ppd0010 crop seq car mcherry2 vector - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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